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實戰(zhàn)分享|小鼠淋巴細(xì)胞的提取和分選之經(jīng)驗小結(jié)
實驗常用人肝癌細(xì)胞株(HepG2/Hep3B,HuH-7,MHCC97H,PLC/PRF/5)怎么選?
巨噬細(xì)胞集落刺激因子(MCSF)重組蛋白
Recombinant Colony Stimulating Factor 1, Macrophage (MCSF)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species | Homo sapiens (Human) |
Product No. | xyA090Hu01 |
Source | Prokaryotic expression |
Host | E.coli |
Purity | > 90% |
UOM | 50ug |
Predicted Molecular Mass | 80.2kDa |
Concentration | n/a |
Applications | SDS-PAGE; WB; ELISA; IP. |
Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
Residues | Glu33~Ser496 with two N-terminal Tags, His-tag and GST-tag |
Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
巨噬細(xì)胞集落刺激因子(MCSF)重組蛋白 SEQUENCES
The sequence of the target protein is listed below.
EEVSEYCS HMIGSGHLQS LQRLIDSQME TSCQITFEFV DQEQLKDPVC YLKKAFLLVQ DIMEDTMRFR DNTPNAIAIV QLQELSLRLK SCFTKDYEEH DKACVRTFYE TPLQLLEKVK NVFNETKNLL DKDWNIFSKN CNNSFAECSS QDVVTKPDCN CLYPKAIPSS DPASVSPHQP LAPSMAPVAG LTWEDSEGTE GSSLLPGEQP LHTVDPGSAK QRPPRSTCQS FEPPETPVVK DSTIGGSPQP RPSVGAFNPG MEDILDSAMG TNWVPEEASG EASEIPVPQG SPSRPGG GSMQTEPARP SNFLSASSPL PASAKGPA DVTGTALPRV GPVRPTGQDW NHTPQKTDHP SALLRDPPEP GSPRISSLRP QGLSNPSTLS AQPQLSRSHS SGSVLPLGEL EGRRSTRDRR SPAEPEGGPA SEGAARPLPR FNSVPLTDTG HERQSEGSFS PQLQES
巨噬細(xì)胞集落刺激因子(MCSF)重組蛋白 USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
巨噬細(xì)胞集落刺激因子(MCSF)重組蛋白 About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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